mab 0026 mab1278 rnd systems trem26 biolegend  (R&D Systems)

Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine

Article Title: Tracking Innate Immune Activation in a Mouse Model of Parkinson's Disease Using TREM1 and TSPO PET Tracers.

doi: 10.2967/jnumed.121.263039

Figure Lengend Snippet: FIGURE 5. Representative 64Cu-TREM1 PET/CT whole-body maximum-intensity-projection images, coronal brain images, and autoradiography of 40-mm-thick coronal mouse brain sections. Dashed out- lines indicate spleen and coronal brain sections. %ID 5 percentage injected dose; ARG 5 autoradio- graphy; C 5 contralateral brain hemisphere; I 5 ipsilateral brain hemisphere; KO 5 knockout; MIP 5 maximum-intensity projection; OHDA 5 hydroxydopamine.

Article Snippet: Mouse TREM1 mAb (R&D Systems) was conjugated with DOTAN-hydroxysuccinimide (Macrocyclics) using metal-free buffers and previously published procedures (25,32).

Techniques: Positron Emission Tomography-Computed Tomography, Autoradiography, Injection, Knock-Out

Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine

Article Title: Tracking Innate Immune Activation in a Mouse Model of Parkinson's Disease Using TREM1 and TSPO PET Tracers.

doi: 10.2967/jnumed.121.263039

Figure Lengend Snippet: FIGURE 6. Quantification of TREM1 PET tracer signal (saline ipsilateral, 2.1 6 0.45; saline contra- lateral, 2.1 6 0.33; isotype control ipsilateral, 1.9 6 0.29; isotype control contralateral, 1.8 6 0.35; TREM1 knockout ipsilateral, 3.1 6 0.57; TREM1 knockout contralateral, 2.8 6 0.97; day 7 6-hydrox- ydopamine ipsilateral, 2.7 6 0.45; day 7 6-hydroxydopamine contralateral, 2.4 6 0.48; day 14 6-hydroxydopamine ipsilateral, 2.7 6 0.23; day 14 6-hydroxydopamine contralateral, 2.2 6 0.43), ex vivo autoradiography (saline, 1.1 6 0.07; isotype control, 1.7 6 1.12; TREM1 knockout, 1.0 6 0.17; day 7 6-hydroxydopamine, 2.2 6 1.07; day 14 6-hydroxydopamine, 3.3 6 0.32), and g-counting quantification (brain: saline, 0.1 6 0.03; isotype control, 0.1 6 0.02; TREM1 knockout, 0.15 6 0.04; day 7 6-hydroxydopamine, 0.16 6 0.04; day 14 6-hydroxydopamine, 0.13 6 0.01; spleen: saline, 4.8 6 0.74; isotype control, 3.3 6 1.2; TREM1 knockout, 3.5 6 1.82; day 7 6-hydroxydopamine, 5.4 6 0.91; day 14 6-hydroxydopamine, 4.3 6 1.32) in saline, isotype control, and 6-hydroxydop- amine mice. All groups were compared with saline control (significance denoted by *); day 7 6-hydroxydopamine and isotype control were compared (significance denoted by #) as well as day 7 6-hydroxydopamine and TREM1 knockout (significance denoted by 1). Data are mean 6 SD per- centage injected dose per gram. */#P , 0.05. **/##/11P , 0.01. %ID 5 percentage injected dose; ARG 5 autoradiography; KO 5 knockout; OHDA 5 hydroxydopamine; ROI 5 region of interest.

Article Snippet: Mouse TREM1 mAb (R&D Systems) was conjugated with DOTAN-hydroxysuccinimide (Macrocyclics) using metal-free buffers and previously published procedures (25,32).

Techniques: Saline, Control, Knock-Out, Ex Vivo, Autoradiography, Injection

Journal: Journal of nuclear medicine : official publication, Society of Nuclear Medicine

Article Title: Tracking Innate Immune Activation in a Mouse Model of Parkinson's Disease Using TREM1 and TSPO PET Tracers.

doi: 10.2967/jnumed.121.263039

Figure Lengend Snippet: FIGURE 7. Flow cytometry analysis of 6-hydroxydopamine and saline brains. (A) Flow cytometry analysis demonstrating frequency of peripheral myeloid (CD45hi [hi = high] CD11b-positive), lymphoid (CD45-positive CD11b-negative), and microglial (CD45int [int= intermediate] CD11b-positive) cells in brains of 6- hydroxydopamine and saline mice 7 d after stereotactic injection (myeloid: day 7 6-hydroxydopamine ipsilateral, 6.1 6 0.72; day 7 6-hydroxydopamine contra- lateral, 0.12 6 0.09; saline ipsilateral, 0.72 6 0.48; saline contralateral, 0.16 6 0.05; lymphoid: day 7 6-hydroxydopamine ipsilateral, 2.0 6 0.71; day 7 6- hydroxydopamine contralateral, 0.21 6 0.18; saline ipsilateral, 1.3 6 0.67; saline contralateral, 0.36 6 0.18; microglia: day 7 6-hydroxydopamine ipsilateral, 51.1 6 8.76; day 7 6-hydroxydopamine contralateral, 55.9 6 12.18; saline ipsilateral, 57.2 6 5.12; saline contralateral, 57.6 6 9.54). (B) Frequency of TREM1- positive myeloid, lymphoid, and microglial cells (TREM1-positive myeloid: day 7 6-hydroxydopamine ipsilateral, 1.6 6 0.73; day 7 6-hydroxydopamine contra- lateral, 0.03 6 0.025; saline ipsilateral, 0.42 6 0.36; saline contralateral, 0.026 6 0.023; TREM1-positive lymphoid: day 7 6-hydroxydopamine ipsilateral, 0.014 6 0.016; day 7 6-hydroxydopamine contralateral, 0.0068 6 0.01; saline ipsilateral, 0.016 6 0.014; saline contralateral, 0.011 6 0.0065; TREM1-positive micro- glia: day 7 6-hydroxydopamine ipsilateral, 0.39 6 0.14; day 7 6-hydroxydopamine contralateral, 0.23 6 0.18; saline ipsilateral, 0.42 6 0.061; saline contralat- eral, 0.45 6 0.22). Data are mean 6 SD percentage total live singlets. **P , 0.01. ***P , 0.001. ****P , 0.0001. OHDA 5 hydroxydopamine.

Article Snippet: Mouse TREM1 mAb (R&D Systems) was conjugated with DOTAN-hydroxysuccinimide (Macrocyclics) using metal-free buffers and previously published procedures (25,32).

Techniques: Flow Cytometry, Saline, Injection